| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.164525 |
| Chromosome: | chromosome 3 |
| Location: | 1736471 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g153700 | FAP347 | Flagellar Associated Protein 347 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGTACCCAGCGTCGCGTCGTCCGCACAC |
| Internal bar code: | TCGAAGCCGTGTCTTTCCAGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 740 |
| LEAP-Seq percent confirming: | 99.7401 |
| LEAP-Seq n confirming: | 4222 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAGCACGAAGAAACGAAA |
| Suggested primer 2: | GGAGGTGACGTTGTGTGATG |