| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.164535 |
| Chromosome: | chromosome 4 |
| Location: | 503928 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217933 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGAGGCAGCAGCAGCATCGGCAGCAGCG |
| Internal bar code: | CACTGAGCACATGCCGTGGGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1017 |
| LEAP-Seq percent confirming: | 98.962 |
| LEAP-Seq n confirming: | 3909 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGGACAGAGATGCAAAGG |
| Suggested primer 2: | CTAGGCTAGCACAACTGCCC |