| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.164550 |
| Chromosome: | chromosome 3 |
| Location: | 6446949 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g194650 | AOA1 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCGGCTGCCCACACGCGGCAGTGATGCG |
| Internal bar code: | CGCCTGCCCTGGCTGGGCCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 261 |
| LEAP-Seq percent confirming: | 99.6618 |
| LEAP-Seq n confirming: | 10314 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCAAACCAGCATGACAGC |
| Suggested primer 2: | GCGTTTGGCTGTGGATATTT |