| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.164578 |
| Chromosome: | chromosome 3 |
| Location: | 5150546 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g182100 | IPK,ITPK1 | (1 of 1) 2.7.1.134 - Inositol-tetrakisphosphate 1-kinase / Inositol-trisphosphate 6-kinase; Inositol 1%252C3%252C4-trisphosphate 5/6-kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGGCGGATAATCGGTGTCGTTAATTCAA |
| Internal bar code: | TATTGTTAGTCTGATGGGATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 524 |
| LEAP-Seq percent confirming: | 99.598 |
| LEAP-Seq n confirming: | 2230 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGAGTCGCAGCTACCTTT |
| Suggested primer 2: | GCTCTAACACCTCCTGGCTG |