| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.164610 |
| Chromosome: | chromosome 13 |
| Location: | 5171536 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g607800 | (1 of 29) IPR013320 - Concanavalin A-like lectin/glucanase domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCCTGGAGGCTAACGCTGGCGGGTTGG |
| Internal bar code: | CGCCGCCGATTCGTAATCTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 690 |
| LEAP-Seq percent confirming: | 99.6432 |
| LEAP-Seq n confirming: | 1117 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGCCACTTCTATGCTTCA |
| Suggested primer 2: | ACTGACTACGTCCACCTCGC |