| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.164612 |
| Chromosome: | chromosome 7 |
| Location: | 3256283 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g334250 | (1 of 6) PTHR12197//PTHR12197:SF138 - SET AND MYND DOMAIN CONTAINING // SET AND MYND DOMAIN-CONTAINING PROTEIN DDB_G0292140 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGATCCGTCCCAGTCCGGCAGCGGAACC |
| Internal bar code: | GTGTGTTGTCGGGGCACGGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 751 |
| LEAP-Seq percent confirming: | 99.3631 |
| LEAP-Seq n confirming: | 468 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAACAGCAAGGAGGAACGCT |
| Suggested primer 2: | ACACGAAGCCGTAGCTGTCT |