| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.164638 |
| Chromosome: | chromosome 1 |
| Location: | 4514235 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g030700 | PTK14 | (1 of 57) 2.7.10.2 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase; Protein tyrosine kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGTTTTGACACGCCCATTCGGCCAGTAA |
| Internal bar code: | AGATCGTTACAGCCGCAGAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 642 |
| LEAP-Seq percent confirming: | 72.7691 |
| LEAP-Seq n confirming: | 88354 |
| LEAP-Seq n nonconfirming: | 33063 |
| LEAP-Seq n unique pos: | 170 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACGGATGATGTGCCTGTG |
| Suggested primer 2: | ATGGAGTCACCACAACGTCA |