| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.164645 |
| Chromosome: | chromosome 17 |
| Location: | 32227 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g696300 | (1 of 1) PTHR12360//PTHR12360:SF1 - NUCLEAR TRANSCRIPTION FACTOR, X-BOX BINDING 1 NFX1 // NF-X1-TYPE ZINC FINGER PROTEIN NFXL1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCCGCATCCCGGTCCACACACACGGGTA |
| Internal bar code: | GGCACAGTGATGGCCTTTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 627 |
| LEAP-Seq percent confirming: | 99.3671 |
| LEAP-Seq n confirming: | 1570 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAGTGCAAGCTATTAGGC |
| Suggested primer 2: | TGGAAGAGATGGTTGGAAGG |