| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.164669 |
| Chromosome: | chromosome_1 |
| Location: | 4674505 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g032200 | FAL3 | Similar to Flagellar Associated Protein FAP187 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGGTGGACAAGCGCAGTACAGTGCCGTATG |
| Internal bar code: | TAGTGTGGCGGGAAACAAAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 148 |
| LEAP-Seq percent confirming: | 99.7664 |
| LEAP-Seq n confirming: | 427 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATTGTGCTGCGTACATCT |
| Suggested primer 2: | TGAGTAGCTGGCTTTGGGAG |