Insertion junction: LMJ.RY0402.164676_4

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.164676
Chromosome:chromosome_13
Location:4602771
Confidence (%):58
Locus systematic id Locus common name Defline Orientation Feature
Cre13.g604050 antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):CTTCACGGAGCAAGTCACCGCCGCCGCGGC
Internal bar code:TCCGTGGATTACATTCCCGGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:420
LEAP-Seq percent confirming:99.384
LEAP-Seq n confirming:484
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GTGAATGAAAGCGCAACGTA
Suggested primer 2:AATCACAATGGGGGTTTCAA