| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.164679 |
| Chromosome: | chromosome 16 |
| Location: | 3169650 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g666100 | (1 of 1) K14168 - cytoplasmic tRNA 2-thiolation protein 1 (CTU1, NCS6) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGGCAACCCGTCGTCCGCACAACCCGCT |
| Internal bar code: | GCTACTTCTCGAGAGCCGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 619 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 740 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTTCCTGTATGGCGATGG |
| Suggested primer 2: | TACGCTAGCTACGATGCCCT |