Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.164686 |
Chromosome: | chromosome_16 |
Location: | 1349966 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g651850 | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTTCATCAGTGTCAGCAACGGCGAGGTGTC |
Internal bar code: | CGGCTACACCCGAAGGGCGACA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 869 |
LEAP-Seq percent confirming: | 95.9641 |
LEAP-Seq n confirming: | 428 |
LEAP-Seq n nonconfirming: | 18 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCTTTGGCAGCTTCAACTTC |
Suggested primer 2: | AGACACCGGTAAGTTGTCCG |