| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.164689 |
| Chromosome: | chromosome 1 |
| Location: | 4029565 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g026250 | AGA1 | Alpha-galactosidase; (1 of 1) 3.2.1.22 - Alpha-galactosidase / Melibiase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCAAACAAAGGCAGCCGCACGACTGCAC |
| Internal bar code: | GCGCGGCCAGATCATCGTGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 534 |
| LEAP-Seq percent confirming: | 86.7704 |
| LEAP-Seq n confirming: | 892 |
| LEAP-Seq n nonconfirming: | 136 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCGTCGAGTCATGACAGT |
| Suggested primer 2: | CTATAAAATGGCTCGCTGCC |