| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.164695 |
| Chromosome: | chromosome_3 |
| Location: | 4763089 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g178100 | Lipoxygenase | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATGGGTGGGCGGGCCAGCACATGCCGCTGT |
| Internal bar code: | CGCGCTCGGCCCTCCAAGTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 866 |
| LEAP-Seq percent confirming: | 96.5766 |
| LEAP-Seq n confirming: | 536 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTACTACCACAACCCCGTG |
| Suggested primer 2: | CACAAGACGTGGATGTGGTC |