| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.164696 |
| Chromosome: | chromosome 3 |
| Location: | 5631813 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g187150 | (1 of 1) IPR001623//IPR027986//IPR027989//IPR028031 - DnaJ domain // T-cell activation inhibitor, mitochondrial // Domain of unknown function DUF4461 // Domain of unknown function DUF4460 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTCGCGCTCACTGCAAGCAGCACAGGA |
| Internal bar code: | GGCGGCAGGAATCATCTGTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 297 |
| LEAP-Seq percent confirming: | 99.9447 |
| LEAP-Seq n confirming: | 1807 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGCAGGATTTTACGGTGT |
| Suggested primer 2: | TCGTTCTATAATGGGGCTGG |