| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.164704 |
| Chromosome: | chromosome 14 |
| Location: | 258028 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g609400 | (1 of 6) 1.8.4.11 - Peptide-methionine (S)-S-oxide reductase / Peptide methionine sulfoxide reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGAAGAGATGAAATTTAGAAGAGATGAT |
| Internal bar code: | GGAATCTATAATTTCTCGCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 421 |
| LEAP-Seq percent confirming: | 99.7677 |
| LEAP-Seq n confirming: | 859 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCTCAGGAGGAGAACGTG |
| Suggested primer 2: | ACTGAGACCACCCCTGAATG |