Insertion junction: LMJ.RY0402.164705_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.164705
Chromosome:chromosome 14
Location:230575
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre14.g609202 Conserved Protein With Tyrosinase Domain; (1 of 1) 3.4.24.7 - Interstitial collagenase / Vertebrate collagenase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCACCTGCAGGCCACGCGCTGGGCCTGAC
Internal bar code:GCGTCTTGAACTGGCCCCGTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:392
LEAP-Seq percent confirming:97.0414
LEAP-Seq n confirming:164
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CCAGGACAAACTAAACCGGA
Suggested primer 2:TATGGGTCTTGATCCCTTCG