Insertion junction: LMJ.RY0402.164709_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.164709
Chromosome:chromosome 9
Location:7831657
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g416650 Nucleotide-diphospho-sugar transferase; (1 of 6) PTHR10994//PTHR10994:SF72 - RETICULON // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCACTGCGGATGCGACCTCCGCGGAGTGC
Internal bar code:AGGTTCTTCCTTAACGGGACTC

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:161
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACACACACACACACGG
Suggested primer 2:AGCTCCTTTACATGCCGCTA