| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.164709 |
| Chromosome: | chromosome 9 |
| Location: | 7831995 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g416650 | Nucleotide-diphospho-sugar transferase; (1 of 6) PTHR10994//PTHR10994:SF72 - RETICULON // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTTTCCCGCCGCTACGGGCGATGTTAGC |
| Internal bar code: | TTAACCCACTAGGTGTTTCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1020 |
| LEAP-Seq percent confirming: | 99.6512 |
| LEAP-Seq n confirming: | 5714 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTCCTTTACATGCCGCTA |
| Suggested primer 2: | ACACACACACACACACACGG |