| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.164712 |
| Chromosome: | chromosome 2 |
| Location: | 2164964 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g089450 | HRP5 | Hydroxyproline-rich glycoprotein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGAGAAGCCCAACCATCGAGAGCGAGGG |
| Internal bar code: | CCACATAGGGAAGACCTAGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 967 |
| LEAP-Seq percent confirming: | 98.4134 |
| LEAP-Seq n confirming: | 4590 |
| LEAP-Seq n nonconfirming: | 74 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCTTTCCGGGCTTCTTCT |
| Suggested primer 2: | CCGACTATGAGTGAGTGCGA |