Insertion junction: LMJ.RY0402.164738_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.164738
Chromosome:chromosome 6
Location:5628751
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g285900 FAP396 (1 of 2) IPR000104//IPR003590 - Antifreeze protein, type I // Leucine-rich repeat, ribonuclease inhibitor subtype; Flagellar Associated Protein 396 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAGCGTGTCAGTAGGAAGACGCCATGGTA
Internal bar code:AATCTTAGTAGACAATGGGAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:408
LEAP-Seq percent confirming:99.4609
LEAP-Seq n confirming:738
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TGTGTGTGTAGCGGAGAAGC
Suggested primer 2:TAACGATTCTTGTGCTTGCG