| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.164760 |
| Chromosome: | chromosome 14 |
| Location: | 1447357 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g617826 | NUOA11 | NADH:ubiquinone oxidoreductase subunit; (1 of 2) PTHR14110//PTHR14110:SF0 - MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22 // MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGGGCTAAGCTAATTCCTTATGTCAAC |
| Internal bar code: | GAAAGGCGCACCATGTCCCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 708 |
| LEAP-Seq percent confirming: | 99.7519 |
| LEAP-Seq n confirming: | 1206 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATATGTGCGAGAGGTTGGC |
| Suggested primer 2: | TCTTCTTACCGATGATGCCC |