Insertion junction: LMJ.RY0402.164808_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.164808
Chromosome:chromosome 9
Location:7036178
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre09.g411250 (1 of 60) IPR001611 - Leucine-rich repeat 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGTGACGCACGCAATTCCCCCCCCCCCCC
Internal bar code:CGAATCGTGGTGCGGGACGGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:522
LEAP-Seq percent confirming:95.9596
LEAP-Seq n confirming:285
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTCACCTCAACTGCAACA
Suggested primer 2:GTTGCGGGAAGGTATTCAAA