Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.164858 |
Chromosome: | chromosome 11 |
Location: | 3024324 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre11.g478600 | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCAGTCGCCTGCTGCTGGCCGTGCGACC |
Internal bar code: | CGATCCTCGAGAGTCCTTCTAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 925 |
LEAP-Seq percent confirming: | 99.591 |
LEAP-Seq n confirming: | 3652 |
LEAP-Seq n nonconfirming: | 15 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCTAGGAAGGCTGGGGAAAT |
Suggested primer 2: | CGCTTTCTGCCTATCTCACC |