| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.164862 |
| Chromosome: | chromosome 3 |
| Location: | 7598576 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g204600 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGCTGGATAGCGCTGGTCTGGACGCCGA |
| Internal bar code: | GAGCGGGTAAAGTGCAGGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 425 |
| LEAP-Seq percent confirming: | 99.474 |
| LEAP-Seq n confirming: | 1702 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTGCCAAGTGCAAAAACC |
| Suggested primer 2: | GCGTCAGCTTTCTTACTGGC |