| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.164887 |
| Chromosome: | chromosome 12 |
| Location: | 9200457 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g541904 | (1 of 1) PF08755//PF13369 - Hemimethylated DNA-binding protein YccV like (YccV-like) // Transglutaminase-like superfamily (Transglut_core2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGCCCGGTCTATTATTGACGCCATTTT |
| Internal bar code: | GTTAATGCTGTAAATAGTAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1629 |
| LEAP-Seq percent confirming: | 99.6072 |
| LEAP-Seq n confirming: | 1268 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCAATGTTGCTACTGCCG |
| Suggested primer 2: | CACCTCTAACGCCTCTCTCG |