| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.164914 |
| Chromosome: | chromosome 7 |
| Location: | 766184 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g317908 | (1 of 1) K04485 - DNA repair protein RadA/Sms (sms, radA) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGTGTGCGCCTGCATGTGCCCCGCCTCC |
| Internal bar code: | GGTTGGGTCACTCTAACTTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 301 |
| LEAP-Seq percent confirming: | 99.7233 |
| LEAP-Seq n confirming: | 5406 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGGAGTTTTGGATCTGGA |
| Suggested primer 2: | GTGGTCTGTTGTTTTGGGCT |