| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.164916 |
| Chromosome: | chromosome 1 |
| Location: | 5143524 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g035800 | IRK2 | (1 of 2) PTHR11767//PTHR11767:SF47 - INWARD RECTIFIER POTASSIUM CHANNEL // SUBFAMILY NOT NAMED; Inwardly-rectifying potassium channel | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAATCTATGCGGCTGACGCTCTTCTATGTC |
| Internal bar code: | CAGTTCCCTATTCTTCCGCCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 772 |
| LEAP-Seq percent confirming: | 99.5082 |
| LEAP-Seq n confirming: | 15985 |
| LEAP-Seq n nonconfirming: | 79 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTAACTTGGCAAGACGG |
| Suggested primer 2: | TAATTCAATGAAGGCTGGGC |