| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.164950 |
| Chromosome: | chromosome 2 |
| Location: | 490685 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g076850 | (1 of 1) PTHR19854:SF1 - GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT BETA-LIKE PROTEIN 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGACCAGCTTATTCTCTTTTGGTCGCTAG |
| Internal bar code: | GGCCGCAGCGGGCCCGGCGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 370 |
| LEAP-Seq percent confirming: | 99.0141 |
| LEAP-Seq n confirming: | 1406 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAAGGAGGAGGCGTACAG |
| Suggested primer 2: | CTCGTCTGTACTCCCTTCGC |