| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.164975 |
| Chromosome: | chromosome_7 |
| Location: | 272149 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g314050 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CACCAGGTAGTCCCACGTCCAGGGGTGGAA |
| Internal bar code: | CTGGGGCGTGCTCTTAGTTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 483 |
| LEAP-Seq percent confirming: | 99.6991 |
| LEAP-Seq n confirming: | 8615 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTCAGCAAGGAGGTGAG |
| Suggested primer 2: | GCAGACTCTAGGAGGCATGG |