| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.164993 |
| Chromosome: | chromosome_12 |
| Location: | 748559 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g495350 | POC12 | Centriole proteome protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTCCGTTCCATCCCTGCCCTCGCCGCCCTC |
| Internal bar code: | ATTGGTTTGATGTAATCAGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 508 |
| LEAP-Seq percent confirming: | 95.4545 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGCATGAGCGGTGAGTAA |
| Suggested primer 2: | GCGTGAGTGGTGATGAGAGA |