Insertion junction: LMJ.RY0402.164993_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.164993
Chromosome:chromosome 12
Location:748560
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g495350 POC12 (1 of 1) K19332 - Meckel syndrome type 1 protein (MKS1); Proteome of centriole protein 12 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGCAGTTTGGGCATGGTGCAGCGTGCGGC
Internal bar code:GGGGCGAATTTTTTACAGTCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:707
LEAP-Seq percent confirming:99.3789
LEAP-Seq n confirming:1760
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GATGCATGAGCGGTGAGTAA
Suggested primer 2:GCGTGAGTGGTGATGAGAGA