| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.165003 |
| Chromosome: | chromosome 12 |
| Location: | 569084 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g492750 | (1 of 5) 3.2.1.1 - Alpha-amylase / Glycogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCGGCTTCGCTGTGTGCTCTTGTTGCGG |
| Internal bar code: | GCGCTCCGCCGGGGGCGGTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 740 |
| LEAP-Seq percent confirming: | 86.6703 |
| LEAP-Seq n confirming: | 3173 |
| LEAP-Seq n nonconfirming: | 488 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGCGTCTGGAGAATGTTG |
| Suggested primer 2: | GGCGCAAGGAATAAGTGTGT |