| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.165005 |
| Chromosome: | scaffold 18 |
| Location: | 114980 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g748947 | (1 of 4) K15285 - solute carrier family 35, member E3 (SLC35E3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCTGATGGTTGGCGGGGATGGATTACAG |
| Internal bar code: | CCCTTTCGGTGCTTCGCCCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 849 |
| LEAP-Seq percent confirming: | 99.53 |
| LEAP-Seq n confirming: | 12070 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTTTGTGAACAAGTGGC |
| Suggested primer 2: | GCGGAGGCTGTAGTCTATGG |