| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.165010 |
| Chromosome: | chromosome 3 |
| Location: | 1198241 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g149600 | CYG53 | (1 of 3) PTHR11920//PTHR11920:SF328 - ADENYLATE AND GUANYLATE CYCLASES // SUBFAMILY NOT NAMED; Adenylate/guanylate cyclase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGTGGATTGGGATTTGGGAATGCTGGGC |
| Internal bar code: | CCCACACGGACCATTTGTTACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 811 |
| LEAP-Seq percent confirming: | 99.1525 |
| LEAP-Seq n confirming: | 351 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAATGTCTGAGGTCGAAG |
| Suggested primer 2: | CGGAACCGAAAAATGACACT |