Insertion junction: LMJ.RY0402.165022_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.165022
Chromosome:chromosome 6
Location:6836288
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g295150 (1 of 4) PF13515 - Fusaric acid resistance protein-like (FUSC_2) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGATGGGGAGTGAAGAGCGATGAGGGGG
Internal bar code:TGTTTGCGTAGTAGCTTATAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:114
LEAP-Seq percent confirming:96.3265
LEAP-Seq n confirming:236
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGTCCACCATTTCAACCTGC
Suggested primer 2:GTGTGTTTGTGTATTGCGGG