| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.165028 |
| Chromosome: | chromosome 13 |
| Location: | 4915281 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g606000 | EFG7 | (1 of 1) PTHR23115//PTHR23115:SF14 - TRANSLATION FACTOR // ELONGATION FACTOR FAMILY PROTEIN; Putative organellar translation elongation factor EFG/EF2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCGGGGCCGGCGGGTTTATGTTGCAGTC |
| Internal bar code: | GTCCGAAGCCGTGGTCAAGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 522 |
| LEAP-Seq percent confirming: | 99.7248 |
| LEAP-Seq n confirming: | 1087 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCAGATCTCAACCAGCAC |
| Suggested primer 2: | GGCTCAACTCTCACTCCCTG |