| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.165046 |
| Chromosome: | chromosome_4 |
| Location: | 1079455 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g216250 | FAP276 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCCTGTTTGCACACAATTACAATAGGCCCA |
| Internal bar code: | TTTAACAGCACTTTCGTCGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1017 |
| LEAP-Seq percent confirming: | 98.7342 |
| LEAP-Seq n confirming: | 390 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAAGGATGGTGTCAAACG |
| Suggested primer 2: | TGTGTGTGTCTTTCCAGCAC |