| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.165046 |
| Chromosome: | chromosome_4 |
| Location: | 1079506 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g216250 | FAP276 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTACCACAAAAGGCTGCACACACAGCGCGC |
| Internal bar code: | ACGGGCGGGGCCTACATCCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 135 |
| LEAP-Seq percent confirming: | 59.469 |
| LEAP-Seq n confirming: | 74121 |
| LEAP-Seq n nonconfirming: | 50517 |
| LEAP-Seq n unique pos: | 92 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAAGGATGGTGTCAAACG |
| Suggested primer 2: | TTGGATGGTGTGTGTGTGTG |