| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.165071 |
| Chromosome: | chromosome 8 |
| Location: | 2419553 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g371301 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCCTCCCTTCCACCGCCCCGTCCCTCCC |
| Internal bar code: | TGACTCACCAACGTTCCCTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 179 |
| LEAP-Seq percent confirming: | 97.3634 |
| LEAP-Seq n confirming: | 2031 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACATGCACACACAAAACA |
| Suggested primer 2: | GTAAGATCCGGTGCAGCAAT |