| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.165097 |
| Chromosome: | chromosome 7 |
| Location: | 1419002 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g322900 | (1 of 1) K13806 - sn1-specific diacylglycerol lipase (DAGL) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGTGTTGCCGATGGTGAGCAGGTAGAAG |
| Internal bar code: | GGGTACAGCCGGGTCTGCTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 712 |
| LEAP-Seq percent confirming: | 98.2044 |
| LEAP-Seq n confirming: | 1422 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAAGGCAGCTCCAGGATTG |
| Suggested primer 2: | ACCTTGCTGAACCATTTTCG |