| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.165113 |
| Chromosome: | chromosome 6 |
| Location: | 1882293 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g263200 | FAP325 | Flagellar Associated Protein 325 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGACCTACCCACGGGCTTCTAGACGCGACC |
| Internal bar code: | CTACTTAGGGAAGTGCGAATGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 155 |
| LEAP-Seq percent confirming: | 99.6705 |
| LEAP-Seq n confirming: | 1815 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGGGATATACAACCAGGG |
| Suggested primer 2: | AGGTCGTATACCACGCAAGG |