| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.165123 |
| Chromosome: | chromosome_7 |
| Location: | 3786399 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g338350 | CPLD50 | Predicted protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TACTCCTAGGCTACAACTTCGCTTCCTCTT |
| Internal bar code: | GCCTCCGCCTCGGGTACTTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 126 |
| LEAP-Seq percent confirming: | 99.7506 |
| LEAP-Seq n confirming: | 1200 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGGTTTTATTGCGTGTTG |
| Suggested primer 2: | CGAGGACTGGGTACTGGGTA |