| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.165151 |
| Chromosome: | chromosome 3 |
| Location: | 7186309 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g208050 | HAD1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCGGGCGGTTCGGGGCGCCCCTGCTCGG |
| Internal bar code: | ACTAATCCGTGCAAGAAAGAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1085 |
| LEAP-Seq percent confirming: | 99.6757 |
| LEAP-Seq n confirming: | 3995 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTAAATGACCTCCGAGGAA |
| Suggested primer 2: | ATCATGTGTAAGGCGAAGGG |