Insertion junction: LMJ.RY0402.165164_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.165164
Chromosome:chromosome 1
Location:2389826
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g013100 PRL10 (1 of 14) PTHR10334 - CYSTEINE-RICH SECRETORY PROTEIN-RELATED; Predicted extracellular protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACAAGAAGTTGCTGTTGGTCTTTGATGG
Internal bar code:AACCGCTTCCATGCCGGGAAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:621
LEAP-Seq percent confirming:99.3495
LEAP-Seq n confirming:12523
LEAP-Seq n nonconfirming:82
LEAP-Seq n unique pos:80

Suggested primers for confirmation by PCR

Suggested primer 1:CCAGGAATCAGAGGGAATCA
Suggested primer 2:ACGTGAAGTCCACCTCCAAC