| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.165169 |
| Chromosome: | chromosome 16 |
| Location: | 2093471 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g657700 | (1 of 2) PF13661 - 2OG-Fe(II) oxygenase superfamily (2OG-FeII_Oxy_4) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATAGCCGACCACGAACGCCCCTGGCGCGT |
| Internal bar code: | CGGTTCCTTGGCCTGCCCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 726 |
| LEAP-Seq percent confirming: | 98.3051 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTGACGGTGCTCATCTAC |
| Suggested primer 2: | CACGATGTGGTACATAGGCG |