| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.165204 |
| Chromosome: | chromosome 12 |
| Location: | 7273011 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g557850 | (1 of 7) IPR000048//IPR027417 - IQ motif, EF-hand binding site // P-loop containing nucleoside triphosphate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCCTTGTTACCTTCCCCTGTACATGCTT |
| Internal bar code: | CAGGGCGCTAGAATTGCACTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 246 |
| LEAP-Seq percent confirming: | 26.1634 |
| LEAP-Seq n confirming: | 759 |
| LEAP-Seq n nonconfirming: | 2142 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCAGCAGCAGCAGTAGAG |
| Suggested primer 2: | ACTCAGTCACGAACACACGC |