| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.165216 |
| Chromosome: | chromosome 1 |
| Location: | 4899500 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g034000 | IPB1 | Importin beta; (1 of 1) K14293 - importin subunit beta-1 (KPNB1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTGTGCAATGGACTTCCCCCTACTCCCT |
| Internal bar code: | CGTAGTTGCGCGTACTTTTTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 485 |
| LEAP-Seq percent confirming: | 99.7122 |
| LEAP-Seq n confirming: | 6582 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGTGTGGACTTGGATTCG |
| Suggested primer 2: | CGGATGAGCTGTGTAGACGA |