| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.165250 |
| Chromosome: | chromosome 8 |
| Location: | 3587898 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g375801 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCAAAGGGTCAGGATGGTGATATCGCCGA |
| Internal bar code: | TTGGGTTACTGGTGGAGCCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 686 |
| LEAP-Seq percent confirming: | 97.0844 |
| LEAP-Seq n confirming: | 6360 |
| LEAP-Seq n nonconfirming: | 191 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCTGTGTACGTGTTTGGT |
| Suggested primer 2: | GTATCGGATGAGGGACTGGA |