| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.165253 |
| Chromosome: | chromosome 12 |
| Location: | 5512660 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g531100 | PBA2 | 20S proteasome beta subunit A2; (1 of 2) K02738 - 20S proteasome subunit beta 1 (PSMB6) | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGCATGGCGGCTGTGCCCCCGAGGAGGG |
| Internal bar code: | GTCAGATCCGTTAGGTGCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 552 |
| LEAP-Seq percent confirming: | 96.0578 |
| LEAP-Seq n confirming: | 731 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGACAAGGATTGCAGGAGC |
| Suggested primer 2: | GACATGACAACACGGTCTGG |